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The analysis of a panel of apoptosis-related proteins revealed that RE did not substantially change the expression of these proteins Supplementary Figure 5. BioMed research international. Protective effects of citrus and rosemary extracts on UV-induced damage in skin cell model and human volunteers. Evid Based Complement Alternat Med. Necrosis studies The release of the intracellular enzyme LDH due to permeabilization of the plasma membrane is a hallmark of necrosis

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However, as necroptosis, a mixed mechanism between necrosis and apoptosis and a caspase-independent form of regulated cell death 23 , 24 , shares some morphologic features with necrosis, this option was also evaluated.

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Nevertheless, this antagonism did not seem to be strong enough to counterbalance the additive or synergic interaction between diterpenes and between di and triterpenes in the whole extract. When the four terpenoids were individually considered, significant antiproliferative activity was observed for each one, in correlation with previously reported data reviewed in In addition, mitochondrial function is closely related to ROS production and control. A cytotoxicity detection kit based on the extracellular detection of lactate dehydrogenase LDH was used to monitor cell integrity.

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